In brief

We develop and apply spectrally resolved microscopy with environment-sensitive fluorescent dyes to characterise local environment of the probes and thereby detect molecular interactions. By custom spectral fitting routines, we can typically resolve spectral shifts as low as 1 nm, which gives us high sensitivity to differences in local molecular environment.

We have applied the technique in various fields:

This project greatly benefits from our fruitful collaboration on the development of environment-sensitive probes, also compatible with super-resolution STED microscopy.

Current project members

Selected publications