Fluorescence microspectroscopy as a tool to study mechanism of nanoparticles delivery into living cancer cells

Zoran Arsov, Iztok Urbančič, Maja Garvas, Daniele Biglino, Ajasja Ljubetič, Tilen Koklič, Janez Štrancar
Biomedical optics express, August 2011; doi: 10.1364/BOE.2.002083 (read more here).

In brief

Here we present a development of fluorescence microspectroscopy (spectral imaging) based on a white light spinning disk confocal microscope with emission wavelength selection by a liquid crystal tunable filter. Spectral contrasting of images was used to localize polymer nanoparticles and cell membranes labeled with fluorophores that have substantially overlapping spectra. In addition, fluorescence microspectroscopy enabled spatially-resolved detection of small but significant effects of local molecular environment on the properties of environment-sensitive fluorescent probe.