Effects of different detachment procedures on viability, nitroxide reduction kinetics and plasma membrane heterogeneity of V-79 cells

Urška Batista, Maja Garvas, Marjana Nemec, Milan Schara, Peter Veranič, Tilen Koklic
Cell Biology International, June 2010; doi: 10.1042/CBI20090276 (read more here).

In brief

We characterized the influence of three different detachment procedures: cell scraping by rubber policeman, trypsinization and a citrate buffer treatment on V-79 cells in the plateau phase of growth (arrested in G1). We found out that cell viability was 91% for trypsin treatment, 85% for citrate treatment and 70% for cell scraping.